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C57BL/6J mice were exposed to e-cig vapor <t>containing</t> <t>nicotine</t> (25 mg/ml), propylene glycol (PG) alone and air as a control (2 hrs/day) and euthanized 2 hrs post-last exposure on day 3. The levels of BMAL1, NR1D1 (REV-ERB α ), and PER2, were measured in lung homogenates by <t>ELISA</t> as per manufacturer’s instructions. Data are shown as mean ± SEM, n = 5–6 per group.
Quantitative Sandwich Elisa Kits Per 2, supplied by MyBiosource Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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C57BL/6J mice were exposed to e-cig vapor <t>containing</t> <t>nicotine</t> (25 mg/ml), propylene glycol (PG) alone and air as a control (2 hrs/day) and euthanized 2 hrs post-last exposure on day 3. The levels of BMAL1, NR1D1 (REV-ERB α ), and PER2, were measured in lung homogenates by <t>ELISA</t> as per manufacturer’s instructions. Data are shown as mean ± SEM, n = 5–6 per group.
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C57BL/6J mice were exposed to e-cig vapor <t>containing</t> <t>nicotine</t> (25 mg/ml), propylene glycol (PG) alone and air as a control (2 hrs/day) and euthanized 2 hrs post-last exposure on day 3. The levels of BMAL1, NR1D1 (REV-ERB α ), and PER2, were measured in lung homogenates by <t>ELISA</t> as per manufacturer’s instructions. Data are shown as mean ± SEM, n = 5–6 per group.
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C57BL/6J mice were exposed to e-cig vapor <t>containing</t> <t>nicotine</t> (25 mg/ml), propylene glycol (PG) alone and air as a control (2 hrs/day) and euthanized 2 hrs post-last exposure on day 3. The levels of BMAL1, NR1D1 (REV-ERB α ), and PER2, were measured in lung homogenates by <t>ELISA</t> as per manufacturer’s instructions. Data are shown as mean ± SEM, n = 5–6 per group.
Quantitative Sandwich Elisa Kits, supplied by Adeza Biomedical Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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C57BL/6J mice were exposed to e-cig vapor <t>containing</t> <t>nicotine</t> (25 mg/ml), propylene glycol (PG) alone and air as a control (2 hrs/day) and euthanized 2 hrs post-last exposure on day 3. The levels of BMAL1, NR1D1 (REV-ERB α ), and PER2, were measured in lung homogenates by <t>ELISA</t> as per manufacturer’s instructions. Data are shown as mean ± SEM, n = 5–6 per group.
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customized quantitative multiplex sandwich elisa kits  (MultiSciences Biotech Co Ltd)
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C57BL/6J mice were exposed to e-cig vapor <t>containing</t> <t>nicotine</t> (25 mg/ml), propylene glycol (PG) alone and air as a control (2 hrs/day) and euthanized 2 hrs post-last exposure on day 3. The levels of BMAL1, NR1D1 (REV-ERB α ), and PER2, were measured in lung homogenates by <t>ELISA</t> as per manufacturer’s instructions. Data are shown as mean ± SEM, n = 5–6 per group.
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C57BL/6J mice were exposed to e-cig vapor <t>containing</t> <t>nicotine</t> (25 mg/ml), propylene glycol (PG) alone and air as a control (2 hrs/day) and euthanized 2 hrs post-last exposure on day 3. The levels of BMAL1, NR1D1 (REV-ERB α ), and PER2, were measured in lung homogenates by <t>ELISA</t> as per manufacturer’s instructions. Data are shown as mean ± SEM, n = 5–6 per group.
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C57BL/6J mice were exposed to e-cig vapor <t>containing</t> <t>nicotine</t> (25 mg/ml), propylene glycol (PG) alone and air as a control (2 hrs/day) and euthanized 2 hrs post-last exposure on day 3. The levels of BMAL1, NR1D1 (REV-ERB α ), and PER2, were measured in lung homogenates by <t>ELISA</t> as per manufacturer’s instructions. Data are shown as mean ± SEM, n = 5–6 per group.
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sandwich elisa kits for quantitation of tnf-α  (EuroClone)
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EuroClone sandwich elisa kits for quantitation of tnf-α
C57BL/6J mice were exposed to e-cig vapor <t>containing</t> <t>nicotine</t> (25 mg/ml), propylene glycol (PG) alone and air as a control (2 hrs/day) and euthanized 2 hrs post-last exposure on day 3. The levels of BMAL1, NR1D1 (REV-ERB α ), and PER2, were measured in lung homogenates by <t>ELISA</t> as per manufacturer’s instructions. Data are shown as mean ± SEM, n = 5–6 per group.
Sandwich Elisa Kits For Quantitation Of Tnf α, supplied by EuroClone, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


C57BL/6J mice were exposed to e-cig vapor containing nicotine (25 mg/ml), propylene glycol (PG) alone and air as a control (2 hrs/day) and euthanized 2 hrs post-last exposure on day 3. The levels of BMAL1, NR1D1 (REV-ERB α ), and PER2, were measured in lung homogenates by ELISA as per manufacturer’s instructions. Data are shown as mean ± SEM, n = 5–6 per group.

Journal: PLoS ONE

Article Title: Waterpipe smoke and e-cigarette vapor differentially affect circadian molecular clock gene expression in mouse lungs

doi: 10.1371/journal.pone.0211645

Figure Lengend Snippet: C57BL/6J mice were exposed to e-cig vapor containing nicotine (25 mg/ml), propylene glycol (PG) alone and air as a control (2 hrs/day) and euthanized 2 hrs post-last exposure on day 3. The levels of BMAL1, NR1D1 (REV-ERB α ), and PER2, were measured in lung homogenates by ELISA as per manufacturer’s instructions. Data are shown as mean ± SEM, n = 5–6 per group.

Article Snippet: The levels of BMAL1, NR1D1, and PER 2 were measured in undiluted lung homogenates of mice exposed to WPS (10-day exposure) and e-cig vapors containing nicotine (3-day exposure) using pre-coated quantitative sandwich ELISA kits according to the instructions from the manufacturer (BMAL-1, Catalog # MBS9328769; NRID1, catalog # MBS9340203; and PER-2, Catalog # MBS9909846, MyBioSource Inc, San Diego, CA, USA).

Techniques: Control, Enzyme-linked Immunosorbent Assay